General Cell Based Assays
Eurofins Discoverx HitHunter cGMP Assay, 10,000 dp (384-well). (90-0039L)
The HitHunter cGMP Assay is a detection kit offering a robust, sensitive and easy-to-use assay to measure intracellular cGMP levels. This product uses EFC technology, and contains ED-tagged cGMP, anti-cGMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cGMP, the anti-cGMP antibody will be sequestered by binding to this untagged cGMP, allowing for EFC between the tagged cGMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit may be used with Eurofins DiscoverX cell lines as well as cell lines from other sources. This detection kit contains reagents for 25 plates (384 well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter eXpress GALR1 Total Internalization Assay, 2pl Kit. (93-0781E3CP5M)
The PathHunter GALR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GALR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged GALR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GALR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress SSTR3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0131E2CP2L)
The cAMP Hunter SSTR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR3, a naturally Gi/Go coupled GPCR. Upon activation of SSTR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress F2RL1 (PAR2; GPR11) U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0235E3CP0L)
The PathHunter F2RL1 (PAR2; GPR11) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, SLIGRL) based activation of F2RL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL1 and EA-tagged β-Arrestin-2. Activation of F2RL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVR1C/ACVR2 Dimerization Assay, 10pl Kit. (93-1069E3CP5L)
The PathHunter ACVR1C/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. activin B) induced dimerization of ACVR1C/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR1C target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1C/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human Cathepsin D ASYQP
The R-PLEX Human Cathepsin D Assay Kit from Meso Scale Discovery is used to quantitatively measure levels of active human Cathepsin D in cell culture supernatants serum plasma or other biological samples This electrochemiluminescent immunoassay utilizes MSD s proprietary SULFO-TAG labels and multi-array plate technology to provide sensitive and rapid detection of Cathepsin D with a lower limit of detection of 19 pg/mL The kit includes a 96-well plate pre-coated with capture antibody detection antibody conjugated to ruthenium label calibrators and controls The assay can be fully automated on MSD instrument platforms and has a total assay time of 3 5 hours
Meso Scale Discovery RPLEX HUM Alphaamylase 1 ASY
The R-PLEX Human Alpha-Amylase 1 Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay for measuring human alpha-amylase 1 in serum plasma saliva and cell culture supernatants It uses electrochemiluminescence (ECL) detection for high sensitivity broad dynamic range and rapid results The kit includes a 96-well plate with capture antibodies ECL-labeled detection antibodies calibrators controls diluent read buffer and wash buffer Sensitivity is 0 08 ng/mL with an upper quantification limit of 1500 ng/mL
Eurofins Discoverx PathHunter eXpress NMBR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0925E2CP2M)
The PathHunter NMBR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuromedin B) based activation of NMBR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NMBR and EA-tagged β-Arrestin-2. Activation of NMBR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL17RA/IL17RC Dimerization Assay, 10-plate Kit. (93-0931E1CP7L)
The PathHunter IL17RA/IL17RC Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-17a) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCXCR6 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0512E2MCP0M)
The PathHunter mCXCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL16) based activation of mCXCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR6 and EA-tagged β-Arrestin-2. Activation of mCXCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR6 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0205E2CP2L)
The PathHunter CXCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL16) based activation of CXCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR6 and EA-tagged β-Arrestin-2. Activation of CXCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mPTGER4 DLD1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0982E13MCP25L)
The PathHunter mPTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of mPTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTGER4 and EA-tagged β-Arrestin-2. Activation of mPTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioss Beta Actin CAEEL/Loading Contr
Beta Actin (CAEEL Loading Control) Primary Antibody Unconjugated Host Species Mouse Target Species Caenorhabditis Elegans Clonality Monoclonal Applications WB Concentration 1ug/ul Size 100 ul Storage Buffer 0 01M TBS(pH7 4) with 1% BSA 0 02% Proclin300 and 50% Glycerol
Eurofins Discoverx PathHunter eXpress GPR4 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0369E2ACP1L)
The PathHunter GPR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR4 and EA-tagged β-Arrestin-2. Activation of GPR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR61 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0407E2ACP1M)
The PathHunter GPR61 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR61 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR61 and EA-tagged β-Arrestin-2. Activation of GPR61 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.